Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi" (INGM)

1 ARTICLES PUBLISHED IN JoVE

Genetics

A Versatile Pipeline for Analyzing Dynamic Changes in Nuclear Bodies in a Variety of Cell Types

Valeria Di Gioia^1,2, Jan Zamporlini^1,3, Rebecca Vadalà^1,3, Elena Parmigiani⁴, Beatrice Bodega^1,3, Federica Marasca¹

¹Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi" (INGM), ²SEMM, European School of Molecular Medicine, ³Department of Biosciences, University of Milan, ⁴T-One Therapeutics Srl

This method describes an immunofluorescence protocol and quantification pipeline for evaluating protein distribution with varied nuclear organization patterns in human T lymphocytes. This protocol provides step-by-step guidance, starting from sample preparation and continuing through the execution of semi-automated analysis in Fiji, concluding with data handling by a Google Colab notebook.