the first affiliated hospital of Sun Yat-sen University

5 ARTICLES PUBLISHED IN JoVE

Cancer Research

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Ruoji Zhou^1,2, An Xu¹, Jian Tu^1,3, Mo Liu¹, Julian A Gingold⁴, Ruiying Zhao¹, Dung-Fang Lee^1,2,5,6

¹Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, ²Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, ³Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, ⁴Women's Health Institute, Cleveland Clinic Foundation, ⁵Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, ⁶Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts.

Immunology and Infection

Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System

Daniel E. Morales-Mantilla^1,2, Xinyan Huang^3,7, Philip Erice^1,3, Paul Porter⁴, Yun Zhang^1,5, Mary Figueroa⁶, Joya Chandra⁶, Katherine Y. King², Farrah Kheradmand^4,8, Antony Rodríguez^3,8

¹Program in Immunology, Baylor College of Medicine, ²Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, ³Department of Medicine - Immunology Allergy and Rheumatology, Baylor College of Medicine, ⁴Department of Medicine, Pulmonary, Critical Care, Sleep Medicine, Baylor College of Medicine, ⁵Department of Pathology and Immunology, Baylor College of Medicine, ⁶Department of Pediatrics, Research and Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, ⁷Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁸Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey VA Medical Center

This protocol demonstrates the study of the pathophysiologic effects of cigarette smoke (CS) with a whole-body inhalation (WBI) exposure system (WBIS) built in-house. This system can expose animals to CS under controlled repeatable conditions for research of CS-mediated effects on lung emphysema and hematopoiesis.

Medicine

Use of the Scissor-Type Knife During the Peroral Endoscopy Myotomy Procedure for the Treatment of Achalasia

Zhen-Zi Huang^*1, Yuan-Qi Li^*1, Hong-Zhan Tian¹, Yi Cui¹, Min-Hu Chen¹, Xiang-Bin Xing¹

¹Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-sen University

To minimize the technical difficulty and improve the safety of peroral endoscopic myotomy (POEM), we describe a protocol for using a scissor-type knife for the main steps of POEM, including mucosal incision, submucosal tunneling, myotomy, and hemostasis.

Medicine

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

Ming-kuan Zhou^*1, Jin-tao Zhuang^*1, Wu-yuan Liao², Shi-ying Long³, Xiang-an Tu¹

¹Department of Urology, The East Division of the First Affiliated Hospital of Sun Yat-Sen University, ²Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, ³Center for Reproductive Medicine, Affiliated Hospital of Guilin Medical University

The present protocol describes a vessel-sparing, longitudinal intussusception vasoepididymostomy using readily available single-needle sutures in China, a safe and effective procedure, which may improve patients' patency and natural pregnancy rates.

Biology

Three-Dimensional Imaging of Aortic Tissues in Atherosclerosis

Ting Sun^1,2,3, Mohammad R. Monjezi², Xu Xu², Changjun Yin^1,2,3,4, Andreas J.R. Habenicht^2,4, Sarajo K. Mohanta^2,4

¹Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, ²Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University, ³Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁴German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance

Here, we present optimized tissue-clearing protocols to image the murine aorta in three dimensions (3D). We delineate state-of-the-art procedures for immunostaining, optical clearing, and imaging with the intent to define the anatomical proximity of the peripheral nervous system with atherosclerotic plaques and the adventitia in atherosclerosis.