Immunometabolic Circuits in Infection for Advancing Host Directed TherapiesShweta Khandibharad 1, Shailza Singh 1
1Systems Medicine Laboratory, Biotechnology Research and Innovation Council - National Centre for Cell Science (BRIC-NCCS)
Here, a protocol for designing, constructing, and delivering tetracycline-controlled based inducible synthetic circuit is presented. The effect of the synthetic circuits on parasite elimination and cytokine expression can be studied by channelizing the presented pipeline. This protocol is relevant for researchers studying synthetic circuit-based therapeutics in infectious diseases.