Sign In

Royal Adelaide Hospital

1 ARTICLES PUBLISHED IN JoVE

image

Biology

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
Elizabeth Vinod 1,2, Ganesh Parasuraman 2, Abel Livingston 3, Solomon Sathishkumar 1, Boopalan Ramasamy 4,5
1Department of Physiology, Christian Medical College, 2Centre for Stem Cell Research (A unit of InStem, Bengaluru), Christian Medical College, 3Department of Orthopedics, Christian Medical College, 4Faculty of Health and Medical Sciences, The University of Adelaide, 5Department of Orthopedics and Trauma, Royal Adelaide Hospital

This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved