Dissection of Mosquito Ovaries, Midgut, and Salivary Glands for Microbiome Analyses at the Organ LevelJordan Tutagata 1, Nicolas Pocquet 2, Blandine Trouche 1, Julie Reveillaud 1
1Infectious Diseases and Vectors: Ecology, Genetics, Evolution and Control (MIVEGEC), University of Montpellier, INRAe, CNRS, IRD, 2Unité de Recherche et d’Expertise en Entomologie Médicale, Institut Pasteur de Nouvelle-Calédonie
Microbial communities in mosquitoes hold great promise for vector biocontrol strategies. Most symbionts are uncultivable, requiring metagenomic analyses. We describe a method to dissect female mosquitoes and separate ovaries, midgut, and salivary glands preventing cross-contamination, facilitating microbiome studies at the organ level, and enhancing understanding of microorganisms' roles in mosquito biology.
