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State University of New York Upstate Medical University

2 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
Xiaowen Wang 1, MacMillan Mbantenkhu 1, Sara Wierzbicki 1, Xin Jie Chen 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

The yeast mitochondrial nucleoid protein, Mgm101, is a Rad52-type recombination protein that forms large oligomeric rings. A protocol is described to prepare soluble recombinant Mgm101 using the Maltose Binding Protein (MBP)-tagging strategy coupled with cation exchange and size exclusion chromatography.

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Neuroscience

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
Arnav Rana 1, Paul T. Massa 2, Xin Jie Chen 1,3
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

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