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University of Tennessee College of Medicine

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
Shengyuan Ding 1, Fu- Ming Zhou 1
1Department of Pharmacology, University of Tennessee College of Medicine

Neurons are first characterized electrophysiologically. Then the cytoplasm from the recorded neuron is aspirated and subjected to reverse transcription-PCR analysis to detect the expression of mRNAs for neurotransmitter synthesis enzymes, ion channels, and receptors.

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