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Hacettepe University

3 ARTICLES PUBLISHED IN JoVE

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Biochemistry

N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection
Haci Mehmet Kayili 1, Bekir Salih 2
1Department of Biomedical Engineering, Karabuk University, 2Department of Chemistry, Hacettepe University

N-glycan profiling of glycoproteins is essential for discovering novel biomarkers and understanding glycan functions in cellular events. Additionally, N-glycan analysis of protein biopharmaceuticals is very important for human use. In this current article, a high-throughput strategy for identifying and quantifying N-glycan structures was presented using the HILIC-FLD-MS/MS technique.

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Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release
Berfin Sucu 1, Ayşe Bayraktar 1,2, Hatice Duman 1, Ayşenur Arslan 1, Merve Kaplan 1, Melda Karyelioğlu 1, Eda Ntelitze 1, Taner Taştekin 1, Seray Yetkin 1, Melih Ertürk 2, Steven A. Frese 3,4, Bethany M. Henrick 4,5, Hacı Mehmet Kayili 6, Bekir Salih 7, Sercan Karav 1
1Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, 2Uluova Dairy Farm, 3Department of Nutrition, University of Nevada, 4Department of Food Science and Technology, University of Nebraska, 5Evolve BioSystems Inc., 6Department of Biomedical Engineering, Karabuk University, 7Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.

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Biochemistry

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Zekiye Ceren Arituluk 1,2, Bishnu Adhikari 1, Urmila Maitra 1, Caroline Goodman 1, Lukasz M. Ciesla 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

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