University Hospital Cologne

2 ARTICLES PUBLISHED IN JoVE

Medicine

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Lukas Andereggen^3,4,5, Volker Neuschmelting^1,6, Michael von Gunten⁷, Hans Rudolf Widmer⁵, Jukka Takala¹, Stephan M. Jakob¹, Javier Fandino^1,2, Serge Marbacher^1,2

¹Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), ²Department of Neurosurgery, Kantonsspital Aarau, ³Laboratories for Neuroscience Research in Neurosurgery, Boston Children's Hospital, ⁴Harvard Medical School, Boston Children's Hospital, ⁵Department of Neurosurgery, University and Bern University Hospital (Inselspital), ⁶Department of Neurosurgery, University Hospital Cologne, ⁷Institute of Pathology, Länggasse Bern

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

Cancer Research

Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

Ningbo Fan^*1, Lisa Raatz^*1, Seung-Hun Chon¹, Alexander Quaas², Christiane Bruns¹, Yue Zhao¹

¹Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, ²Institute of Pathology, University Hospital Cologne

This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.