JoVE Logo

Sign In

DeSales University

2 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits
Joshua B. Slee 1,2, Ivan S. Alferiev 1,2, Robert J. Levy 1,2, Stanley J. Stachelek 1,2
1Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, 2University of Pennsylvania Perelman School of Medicine

Blood exposure to polymeric blood conduits initiates the foreign body reaction that has been implicated in clinical complications. Here, the Chandler Loop Apparatus, an experimental tool mimicking blood perfusion through these conduits, is described. Appendage of recombinant CD47 results in decreased evidence of the foreign body reaction on these conduits.

image

Genetics

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Sara Lynn N. Farwell 1, Joshua B. Slee 2, Yaqiu Li 1, Linda J. Lowe-Krentz 1
1Department of Biological Sciences, Lehigh University, 2Department of Natural Science, DeSales University

A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved