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Shepherd University

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Functional Cloning Using a Xenopus Oocyte Expression System
Carol Zygar Plautz 1, Hannah C. Williams 1, Robert M. Grainger 2
1Department of Biology, Shepherd University, 2Department of Biology, University of Virginia

We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.

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