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Louisiana Tech University

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP
Sonia G. Parra *1, Sam S. Vesuna *1, Teresa A. Murray 1,2, Michael J. Levene 1
1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University

Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.

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Bioengineering

Generation of Scalable, Metallic High-Aspect Ratio Nanocomposites in a Biological Liquid Medium
Kinsey Cotton Kelly 1, Jessica R. Wasserman 2, Sneha Deodhar 3, Justin Huckaby 4, Mark A. DeCoster 4,5
1Biophysics Department, Centenary College of Louisiana, 2Department of Chemistry, Louisiana Tech University, 3Department of Integrative Physiology, University of North Texas Health Sciences Center, 4Biomedical Engineering, Louisiana Tech University, 5Institute for Micromanufacturing, Louisiana Tech University

Here we present a protocol to synthesize novel, high-aspect ratio biocomposites under biological conditions and in liquid media. The biocomposites scale from nanometers to micrometers in diameter and length, respectively. Copper nanoparticles (CNPs) and copper sulfate combined with cystine are the key components for the synthesis.

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Biology

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Luke A. White 1, Emily V. Stevenson 1, J. Winny Yun 1, Randa Eshaq 1, Norman R. Harris 1, David K. Mills 2, Alireza Minagar 3, Pierre-Olivier Couraud 4, J. Steven Alexander 1
1Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences, Louisiana Tech University, 3Neurology, Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

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JoVE Journal

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
Thea M. Edwards 1,2, Howard E. Morgan 2,3, Coralia Balasca 4, Naveen K. Chalasani 5, Lauren Yam 4,6, Alison M. Roark 4
1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University

This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.

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