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Northwest University

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
Bingchen Che *1, Jie Zhu *1, Dan Sun 1, Xiaoqiang Feng 1, Ce Zhang 1
1State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University

We present a microfluidic system for high throughput studies on complex life machinery, which consists of 1500 culture units, an array of enhanced peristaltic pumps and an on-site mixing modulus. The microfluidic chip allows for the analysis of the highly complex and dynamic micro-environmental conditions in vivo.

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Genetics

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
Clarisse van der Feltz 1
1College of Arts and Sciences, Northwest University

The ACT1-CUP1 assay, a copper growth assay, provides a quick readout of precursor messenger RNA (pre-mRNA) splicing and the impact mutant splicing factors have on spliceosomal function. This study provides a protocol and highlights the customization possible to address the splicing question of interest.

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Bioengineering

Isolation and Culture of Bone Marrow Mesenchymal Stem Cells from the Human Mandible
Chunlin Zong *1, Lu Zhao *1, Chong Huang 2, Yuanli Chen 1, Lei Tian 1
1State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, 2College of Life Sciences, Northwest University

The present protocol describes an efficient procedure for isolating and culturing of human mandibular bone marrow-derived mesenchymal stem cells using the whole bone marrow adherence method. The cultured cells were identified by cell proliferation assays, flow cytometry, and multilineage differentiation induction.

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Biology

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
Yuan Cao *1,2, Ji-Yu Qiu *1,3, Da Chen 1,2, Chen-Yao Li 1,4, Shu-Juan Xing 1,5, Chen-Xi Zheng 1, Xin Liu 2, Yan Jin 1,6, Bing-Dong Sui 1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

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Biology

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
Shu-Juan Xing 1,2, Kai-Chao Zhang 2,3, Si-Yuan Tang 2,4, Lu Liu 2,5, Yuan Cao 2,5, Chen-Xi Zheng 2, Bing-Dong Sui 2, Yan Jin 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

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Environment

A Complete Method for Evaluating the Performance of Photocatalysts for the Degradation of Antibiotics in Environmental Remediation
Bing Wang 1, XuXia Zhang 1, LeCheng Li 1, MengTing Ji 1, Zheng Zheng 1, ChuanHui Shi 1, Zhuo Li 1, Hong Hao 1
1School of Chemical Engineering, Northwest University

Presented here is a protocol to explore a universal set of experimental procedures for comprehensive laboratory evaluation of photocatalysts in the field of environmental purification, using the example of photocatalytic removal of antibiotic organic pollutant molecules from water by phthalocyanine sensitized silver phosphate composites.

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Bioengineering

Construction, Characterization, and Regenerative Application of Self-Assembled Human Mesenchymal Stem Cell Aggregates
Shijie Li 1,2, Peisheng Liu 1,2,3, Ting Zhu 1,2,3, Bingdong Sui 1, Yan Jin 1, Kun Xuan 2, Chenxi Zheng 1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, The Fourth Military Medical University, 2Department of Preventive Dentistry, School of Stomatology, The Fourth Military Medical University, 3College of Life Science, Northwest University

This study describes a method to construct aggregates based on the self-assembly of human mesenchymal stem cells and identifies the morphological and histological characteristics for the regenerative treatment of cranial bone defects.

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