Comparative Karyotype Analysis of Lycoris aurea Herb Populations Using Fluorochrome Banding and 45S and 5S rDNA-FISHXianghui Jiang 1, Miaohua Quan 1, Chaowen She 2
1Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, 2College of Biological and Food Engineering, Hunan University
This protocol investigates the heterochromatin distribution in eight populations of Lycoris aurea (L′Her) Herb, using combined PI and DAPI chromosome staining, fluorescence in situ hybridization (FISH) with 18S-5.8-26S rRNA gene regions (45S rDNA regions), and 5S ribosomal DNA probes.
