Hunan Provincial Maternal and Child Health Care Hospital

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Proximity Ligation Assay to Study Oncogene-Derived Transcription-Replication Conflicts

Linling Ke¹, Qian Xie¹, Xiaoman Wang¹, Yuanhang Gong¹, Min Li^1,2

¹Institute of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, ²National Health Commission Key Laboratory of Birth Defect Research and Preventio, Hunan Provincial Maternal and Child Health Care Hospital

Here, we present a sensitive and specific method to detect oncogene-induced transcription-replication conflicts (TRCs) using the proximity ligation assay (PLA). This approach leverages specific antibodies targeting PCNA and phospho-CTD RNAPII, enabling the assessment of TRC prevalence between RNA polymerase II transcription and DNA replication machinery.