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University Medical Center Hamburg-Eppendorf (UKE)

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
Kai Januschowski 1, Sebastian Müller 1, Carlo Krupp 1, Martin S. Spitzer 1, José Hurst 1, Maximilian Schultheiss 1, Karl-Ulrich Bartz-Schmidt 1, Peter Szurman 1, Sven Schnichels 1
1Centre for Ophthalmology, University Eye Hospital Tübingen

With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.

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Medicine

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
Daniel A. Wenzel 1, Berenike C. Kunzmann 2, Nils A. Steinhorst 1, Martin S. Spitzer 1, Maximilian Schultheiss 1
1Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), 2University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen

Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.

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Immunology and Infection

Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues
Lavinia Schoenfeld 1, Birgit Appl 1, Laia Pagerols-Raluy 1, Annika Heuer 3, Konrad Reinshagen 1, Michael Boettcher 1,2
1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, University of Hamburg, 2Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, 3Division of Spine Surgery, Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf (UKE)

Neutrophil extracellular traps (NETs) are associated with various diseases, and immunofluorescence is often used for their visualization. However, there are various staining protocols, and, in many cases, only one type of tissue is examined. Here, we establish a generally applicable protocol for staining NETs in mouse and human tissue.

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