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University of Salzburg

2 ARTICLES PUBLISHED IN JoVE

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JoVE Core

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Markus Steiner 1, Andrea Harrer 2, Roland Lang 3, Michael Schneider 4, Fátima Ferreira 5, Thomas Hawranek 3, Martin Himly 1
1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

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Immunology and Infection

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
Mario Wenger 1, Athanasios Bethanis 1, Litty Johnson 1, Lorenz Aglas 1
1Department of Biosciences, University of Salzburg

Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.

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