Earthworm, Lumbricus Terrestris: A Novel Microinjection Vasculature In vivo Invertebrate ModelAsis Lopez 1,5, Yaswitha Mikkilineni 2, Shayna Berman 3, Damir Khismatullin 1,4, Gregory T. Clement 5, Matthew R. Myers 5
1Bioinnovation PhD Program, Tulane University, 2Biology Department, Virginia Commonwealth University, 3Mechanical Engineering Department, Montgomery College, 4Biomedical Engineering Department, Tulane University, 5US Food and Drug Administration
Earthworms are a novel invertebrate in vivo bench-top model for vasculature studies. We present techniques and equipment that allow efficient surgery and microinjection into the earthworm vasculature. Surgical protocols, microinjection techniques and the procedure for producing custom-made micropipettes are described.
