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University of Lübeck

6 ARTICLES PUBLISHED IN JoVE

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Medicine

A Human Fallopian Tube Model for Investigation of C. trachomatis Infections
Stefan Jerchel 1, Gudrun Knebel 2, Peter König 2, Michael K. Bohlmann 3, Jan Rupp 1,4
1Institute of Medical Microbiology and Hygiene, University of Lübeck, 2Institute of Anatomie, University of Lübeck, 3Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, University of Lübeck, 4Medical Clinic III, University Hospital of Schleswig-Holstein, University of Lübeck

We describe an ex vivo infection model for visualisation of direct interactions from bacterial pathogens with human fallopian tube cells. The whole organ tissue model was established to investigate C. trachomatis induced pathology to the female fallopian tube under "life-like" conditions.

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Bioengineering

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
Thilo L. Schenck 1, Myra N. Chávez 1, Alexandru P. Condurache 2, Ursula Hopfner 1, Farid Rezaeian 3, Hans-Günther Machens 1, José T. Egaña 1,4
1Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, 2Institute for Signal Processing, University of Lübeck, 3Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, 4FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

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Engineering

Robotic Sensing and Stimuli Provision for Guided Plant Growth
Mostafa Wahby 1, Mary Katherine Heinrich 1,5, Daniel Nicolas Hofstadler 2, Julian Petzold 1, Igor Kuksin 3, Payam Zahadat 2,4, Thomas Schmickl 2, Phil Ayres 5, Heiko Hamann 1
1Institute of Computer Engineering, University of Lübeck, 2Institute of Biology, Artificial Life Lab, University of Graz, 3Cybertronica UG, 4Department of Computer Science, IT University of Copenhagen, 5Centre for IT and Architecture, Royal Danish Academy

Distributed robot nodes provide sequences of blue light stimuli to steer the growth trajectories of climbing plants. By activating natural phototropism, the robots guide the plants through binary left-right decisions, growing them into predefined patterns that by contrast are not possible when the robots are dormant.

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Medicine

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss
Annekatrin Holzhey *1, Svenja Sonntag *2, Johannes Rendenbach 1, Justus S. Ernesti 1, Vinodh Kakkassery 2, Salvatore Grisanti 2, Fred Reinholz 3, Sebastian Freidank 3, Alfred Vogel 3, Mahdy Ranjbar 1,2
1Laboratory for Angiogenesis and Ocular Cell Transplantation, University of Lübeck, 2Department of Ophthalmology, University of Lübeck, 3Institute of Biomedical Optics, University of Lübeck

Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK).

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Medicine

Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and Metastasis
Rüdiger Braun 1, Olha Lapshyna 1, Susanne Eckelmann 2,3, Kim Honselmann 1, Louisa Bolm 1, Meike ten Winkel 1, Steffen Deichmann 1, Oliver Schilling 4, Charli Kruse 2,3, Tobias Keck 1, Ulrich Wellner 1, Peter Bronsert 4,5,6, Matthias Brandenburger 3
1Department of Surgery, University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology, University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg, Medical Center - University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg, Medical Center - University of Freiburg

This protocol describes the preparation of organotypic slice cultures (OTSCs). This technique facilitates the ex vivo cultivation of intact multicellular tissue. OTSCs can be used immediately to test for their respective response to drugs in a multicellular environment.

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Neuroscience

Topographical EEG Recordings of Visual Evoked Potentials in Mice using Multichannel Thin-film Electrodes
Rüdiger Land 1, Sarah Christine Sentis 1,2, Andrej Kral 1
1Institute for Audioneurotechnology, Department of Experimental Otology, Hannover Medical School, 2Institute for Endocrinology and Diabetes, Center of Brain, Behavior and Metabolism, University of Lübeck

The present protocol describes a simple procedure to acquire and analyze the topography of epicranial visual evoked potentials with 32-multichannel thin-film electrodes in the mouse.

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