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University of South Australia

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
Maurizio Costabile 1
1School of Pharmacy and Medical Sciences, University of South Australia

Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.

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Immunology and Infection

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
Maurizio Costabile 1
1School of Pharmacy and Medical Sciences, University of South Australia

The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.

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Medicine

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
Benson U.W. Lei 1,2, Miko Yamada 1, Van L.T. Hoang 3, Paul J. Belt 4, Mark H. Moore 5, Lynlee L. Lin 2, Ross Flewell-Smith 1, Nhung Dang 1,2, Shoko Tomihara 2, Tarl W. Prow 1,2
1Future Industries Institute, University of South Australia, 2Faculty of Medicine, University of Queensland, 3Faculty of Health, Queensland University of Technology, 4Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, 5Australian Craniofacial Unit, Women's and Children's Hospital

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.

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