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Davidson College

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Sebastian Flores 1, Andrew P. Feld 1, Bryan H. Thurtle-Schmidt 1
1Department of Biology, Davidson College

Here we present a protocol to express, solubilize, and purify several eukaryotic borate transporters with homology to the SLC4 transporter family using yeast. We also describe a chemical cross-linking assay to assess the purified homomeric proteins for multimeric assembly. These protocols can be adapted for other challenging membrane proteins.

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