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INSTITUTO POLITÉCNICO NACIONAL

4 ARTICLES PUBLISHED IN JoVE

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Environment

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Claudia Guerrero-Barajas 1, Alberto Ordaz 1, Selene Montserrat García-Solares 1, Claudio Garibay-Orijel 1, Fernando Bastida-González 2, Paola Berenice Zárate-Segura 1
1Bioprocesses Department, Laboratory of Environmental Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, 2Laboratory of Molecular Biology, Escuela Superior de Medicina, Instituto Politécnico Nacional

Microbial sulfate reduction is a process of great importance in environmental biotechnology. The success of the sulfidogenic reactors depends among other factors on the microbial composition of the sludge. Here, we present a protocol to develop sulfidogenic sludge from hydrothermal vents sediments in a UASB reactor for reductive dechlorination purposes.

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Chemistry

Determining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation
Monika S. Walczak 1, Perla Morales-Gil 1,2, Turia Belashehr 1, Kiran Kousar 1, Paulina Arellanes Lozada 1,3, Robert Lindsay 1
1Corrosion and Protection Centre, School of Materials, The University of Manchester, 2Laboratorio de Caracterización de Materiales Sintéticos y Naturales, Instituto Mexicano del Petróleo, 3Departamento de Metalurgia y Materiales, Instituto Politécnico Nacional

A protocol to avoid the oxidation of metallic substrates during sample transfer from an inhibited acidic solution to an X-ray photoelectron spectrometer is presented.

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Biochemistry

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
Lucero Romero-Aguilar *1,2, Mónica Montero-Lomeli *3, Juan Pablo Pardo 2, Guadalupe Guerra- Sánchez 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro

Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis.

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Medicine

Evaluation of Hydration Status by Bioelectrical Impedance Vector Analysis in Patients with Ischemic Heart Disease Undergoing Exercise Stress Test
Pineda-Juárez Juan Antonio 1, Navarrete-de la O Dulce María 1, Suárez-Cuenca Juan Antonio 2, Lara-Vargas Jorge Antonio 3, Arteaga-Martínez José Rodolfo 3, Radillo-Alba Hugo Alberto 3, Leyva-Valadez Eduardo Alfonso 3, Morales-Portano Julieta Danira 4, Gómez-Álvarez Enrique 4, Santellano-Juárez Brenda 5, Mondragón-Terán Paul 6
1Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research, Centro Médico Nacional “20 de Noviembre”, ISSSTE, 2Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Centro Médico Nacional “20 de Noviembre”, ISSSTE, 3Cardiac Rehabilitation Service, Centro Médico Nacional “20 de Noviembre”, ISSSTE, 4Cardiology Service, Centro Médico Nacional “20 de Noviembre“, ISSSTE, 5Georgia Cancer Center, 6Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, CICATA-Unidad Morelos, Instituto Politécnico Nacional

Hydration status imbalances can have a short-term effect on direct and indirect determinants of oxygen uptake and pulse, and morbidity and mortality prognostic factors in ischemic heart disease. This protocol describes the technique for assessment of hydration status through bioelectrical impedance vector analysis and cardiopulmonary response during exercise stress test.

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