JoVE Logo

Sign In

The University of Kansas School of Medicine

3 ARTICLES PUBLISHED IN JoVE

image

Biology

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
Randal Halfmann 1,2,3, Susan Lindquist 1,2,3
1Whitehead Institute for Biomedical Research, 2Department of Biology, MIT - Massachusetts Institute of Technology, 3Howard Hughes Medical Institute

SDD-AGE is a useful technique for the detection and characterization of amyloid-like polymers in cells. Here we demonstrate an adaptation that makes this technique amenable to large-scale applications.

image

Neuroscience

Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
Limei Ma 1, Sachiko Haga-Yamanaka 1, Qingfeng Elden Yu 1, Qiang Qiu 1, SangSeong Kim 1, C. Ron Yu 1,2
1Stowers Institute for Medical Research, 2Department of Anatomy and Cell Biology, The University of Kansas School of Medicine

The vomeronasal organ (VNO) detects intraspecies chemical signals that convey social and reproductive information. We have performed Ca2+ imaging experiments using transgenic mice expressing G-CaMP2 in VNO tissue. This approach allows us to analyze the complicated response patterns of the vomeronasal neurons to large numbers of pheromone stimuli.

image

Biology

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
Shriram Venkatesan *1, Tejbir S. Kandola *1, Alejandro Rodríguez-Gama 1, Andrew Box 1, Randal Halfmann 1,2
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine

This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved