Seattle Children's Research Institute
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Emily A. Brown 1,2, Steven C. Neier 3,4, Claudia Neuhauser 5, Adam G. Schrum 6,7,8, Stephen E.P. Smith 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington
Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.
Immunology and Infection
Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
Suhavi Kaur 1, Dana E. Miller 1, Matthew H. Harband 1, Hazem F. M. Abdelaal 1, Susan L. Baldwin 1, Sasha E. Larsen 1, Rhea N. Coler 1,2,3
1Center for Global Infectious Disease Research, Seattle Children's Research Institute, 2Department of Pediatrics, University of Washington School of Medicine, 3Department of Global Health, University of Washington
Here we present a protocol for performing a low-dose intradermal inoculation of Mycobacterium avium in a mouse ear to reflect exposure-like conditions.