JoVE Logo

Sign In

University of Tennessee Health Science Center, Memphis, TN

1 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
Joshua N. Douglas 1,2,3, Lidia A. Gardner 1,2, Sangmin Lee 1,2, Yoojin Shin 1,2, Chassidy J. Groover 1,2, Michael C. Levin 1,2,3
1Research Service, Veterans Administration Medical Center, Memphis, TN, 2Department of Neurology, University of Tennessee Health Science Center, Memphis, TN, 3Department of Anatomy/Neurobiology, University of Tennessee Health Science Center, Memphis, TN

A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved