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United States Geological Survey

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens
Hana T. Millen 1, Jordan C. Gonnering 1, Ryan K. Berg 2, Susan K. Spencer 3, William E. Jokela 3, John M. Pearce 4, Jackson S. Borchardt 1, Mark A. Borchardt 3
1Wisconsin Water Science Center, United States Geological Survey, 2University of Wisconsin – Madison, 3Agricultural Research Service, United States Department of Agriculture, 4Alaska Science Center, United States Geological Survey

Glass wool filters have been used to concentrate waterborne viruses by a number of research groups around the world. Here we show a simple approach for constructing glass wool filters and demonstrate the filters are also effective in concentrating waterborne viral, bacterial and protozoan pathogens.

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Biology

Standardizing a Non-Lethal Method for Characterizing the Reproductive Status and Larval Development of Freshwater Mussels (Bivalvia: Unionida)
Caitlin E. Beaver 1, Susan R. Geda 2, Nathan A. Johnson 1
1Wetland and Aquatic Research Center, United States Geological Survey, 2Garcon Point Aquatic Research Center, Florida Fish and Wildlife Conservation Commission

Freshwater mussel conservation is dependent on monitoring reproductive patterns and processes of species. This study standardizes a non-lethal protocol for sampling gill contents, characterizing larval development, and providing a digital repository for data collected. This protocol-database package will be an important tool for mussel researchers in recovery of imperiled species.

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