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University Hospital Center and University of Lausanne

3 ARTICLES PUBLISHED IN JoVE

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Medicine

How to Measure Cortical Folding from MR Images: a Step-by-Step Tutorial to Compute Local Gyrification Index
Marie Schaer 1, Meritxell Bach Cuadra 2,3, Nick Schmansky 4, Bruce Fischl 4, Jean-Philippe Thiran 2, Stephan Eliez 1
1Department of Psychiatry, University of Geneva School of Medicine, 2Signal Processing Laboratory, École Polytechnique Fédérale de Lausanne, 3Department of Radiology, University Hospital Center and University of Lausanne, 4Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital

Measuring gyrification (cortical folding) at any age represents a window into early brain development. Hence, we previously developed an algorithm to measure local gyrification at thousands of points over the hemisphere1. In this paper, we detail the computation of this local gyrification index.

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Immunology and Infection

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Nicolas Jacquier 1, Sébastien Aeby 1, Julia Lienard 1, Gilbert Greub 1
1Institute of Microbiology, University Hospital Center and University of Lausanne

Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

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Immunology and Infection

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Antony Croxatto 1, Guy Prod'hom 1, Christian Durussel 1, Gilbert Greub 1
1Institute of Microbiology, University Hospital Center and University of Lausanne

A rapid bacterial pellet preparation from a positive blood culture can be used as a sample for applications such as identification by MALDI-TOF, Gram staining, antibiotic susceptibility testing and PCR-based test. The results can be rapidly communicated to clinicians to improve the outcome of patients suffering from bloodstream infections.

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