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Goethe University Hospital Frankfurt

2 ARTICLES PUBLISHED IN JoVE

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Medicine

The CYP2D6 Animal Model: How to Induce Autoimmune Hepatitis in Mice
Edith Hintermann 1, Janine Ehser 1, Urs Christen 1
1Pharmazentrum Frankfurt / ZAFES, Goethe University Hospital Frankfurt

Infection of mice with an Adenovirus expressing the major human autoantigen cytochrome P450 2D6 (hCYP2D6) recognized by sera of patients suffering from type 2 autoimmune hepatitis results in a persistent form of autoimmune-mediated liver disease characterized by extensive hepatitis, fibrosis and generation of a CYP2D6-specific immune response.

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Immunology and Infection

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
Julia Barthelmes *1, Nadja Tafferner *2, Jennifer Kurz 2, Natasja de Bruin 2, Michael J. Parnham 2, Gerd Geisslinger 1, Susanne Schiffmann 2
1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.

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