University of Maribor

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Marko Milojević¹, Boštjan Vihar^1,2, Luka Banović², Mihael Miško², Lidija Gradišnik¹, Tanja Zidarič¹, Uroš Maver^1,3

¹Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, ²Institute for Development of Advanced Applied Systems (IRNAS), ³Department of Pharmacology, Faculty of Medicine, University of Maribor

Presented here is a simple-to-use, core/shell, three-dimensional bioprinting set-up for one-step fabrication of hollow scaffolds, suitable for tissue engineering of vascular and other tubular structures.

Biology

Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Andraž Stožer¹, Jurij Dolenšek^1,2, Lidija Križančić Bombek¹, Viljem Pohorec¹, Marjan Slak Rupnik^1,3, Maša Skelin Klemen¹

¹Institute of Physiology, Faculty of Medicine, University of Maribor, ²Faculty of Natural Sciences and Mathematics, University of Maribor, ³Center for Physiology and Pharmacology, Medical University of Vienna

We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.

Chemistry

Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS

Matjaž Rantaša¹, David Majer¹, Matjaž Finšgar¹

¹Faculty of Chemistry and Chemical Engineering, University of Maribor

The presented protocol describes sample homogenization with a laboratory mixer, acid digestion of food samples using a mixture of 68 wt% HNO₃ and 30 wt% H₂O₂ via microwave-assisted wet acid digestion, and multi-element determination performed with inductively coupled plasma mass spectrometry.