University of Maryland School of Pharmacy

2 ARTICLES PUBLISHED IN JoVE

Biology

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Karthika Natarajan^1,2, Yi Xie^1,3, Takeo Nakanishi⁴, Rebecca S. Moreci^5,6, Pancharatnam Jeyasuria⁷, Arif Hussain^1,3,8,9, Douglas D. Ross^{1,3,8,9,10,11}

¹Greenebaum Cancer Center, University of Maryland School of Medicine, ²Pharmaceutical Sciences, University of Maryland School of Pharmacy, ³Baltimore VA Medical Center, ⁴Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, ⁵Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, ⁶Magee Women's Research Institute, ⁷Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, ⁸Medicine, University of Maryland School of Medicine, ⁹Pathology, University of Maryland School of Medicine, ¹⁰Pharmacology, University of Maryland School of Medicine, ¹¹Experimental Therapeutics, University of Maryland School of Medicine

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

Medicine

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport

Jessica Felton¹, Kunrong Cheng², Anan Said², Aaron C. Shang², Su Xu³, Diana Vivian⁴, Melissa Metry⁵, James E. Polli⁵, Jean-Pierre Raufman^2,6

¹Department of Surgery, University of Maryland School of Medicine, ²Department of Medicine, University of Maryland School of Medicine, ³Department of Radiology, University of Maryland School of Medicine, ⁴Food and Drug Administration, ⁵Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, ⁶VA Maryland Health Care System

Tools to diagnose bile acid malabsorption and measure bile acid transport in vivo are limited. An innovative approach in live animals is described that utilizes combined proton (¹H) plus fluorine (¹⁹F) magnetic resonance imaging; this novel methodology has translational potential to screen for bile acid malabsorption in clinical practice.