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National Institute of Neurological Disorders and Stroke, National Institutes of Health

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
Dvir Blivis 1, Michael J. O'Donovan 1
1Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

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Neuroscience

Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
Maria Chiara G. Monaco 1, Dragan Maric 2, Alexandra Bandeian 1, Emily Leibovitch 1, Wan Yang 1, Eugene O. Major 1
1Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth factors.

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Developmental Biology

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
Tongguang Wang 1, Elliot Choi 1, Maria Chiara G. Monaco 2, Eugene O. Major 2, Marie Medynets 1, Avindra Nath 1
1Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health

A method was developed to directly derive human neural stem cells from hematopoietic progenitor cells enriched from peripheral blood cells.

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Bioengineering

Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells
Angela Ballesteros 1, Kenton J. Swartz 1
1Molecular Physiology and Biophysics Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

Here, we present a method for visualizing the uptake of 3 kDa Texas Red-labeled dextran in auditory hair cells with functional mechanotransduction channels. In addition, dextrans of 3–10 kDa can be used to study endocytosis in hair and supporting cells of the organ of Corti.

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