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BC Cancer Research Centre

3 ARTICLES PUBLISHED IN JoVE

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Biology

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Jennifer Y. Kennett 1, Spencer K. Watson 1, Heather Saprunoff 2, Cameron Heryet 3, Wan L. Lam 1
1Department of Cancer Genetics, BC Cancer Research Centre, 2Deeley Research Centre, BC Cancer Agency, 3Photography/Video Production, Multi-Media Services, BC Cancer Agency

This video is a technical demonstration of the hybridization protocol for whole genome tiling path array CGH, which scans the entire human genome using only 25-100 ng of DNA that can be isolated from a variety of sources, including archival formalin fixed material.

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Biology

Methylated DNA Immunoprecipitation
Kelsie L. Thu *1,2, Emily A. Vucic *1,3, Jennifer Y. Kennett 1,3, Cameron Heryet 5, Carolyn J. Brown 5, Wan L. Lam 1,2,3, Ian M. Wilson 1,3
1Department of Cancer Genetics and Developmental Biology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Department of Pathology and Laboratory Medicine, University of British Columbia - UBC, 4Photography/Video Production, Multi-Media Services, BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,, University of British Columbia - UBC

This video demonstrates the protocol for methylated DNA immunoprecipitation (MeDIP). MeDIP is a two day procedure that selectively extracts methylated DNA fragments from a genomic DNA sample using antibodies with specificity for 5 -methylcytosine (anti-5 mC).

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Biology

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Larissa A. Pikor *1,2, Katey S. S. Enfield *1,2, Heryet Cameron 3, Wan L. Lam 1,2,4
1Department of Integrative Oncology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Photography/Video Production, Multi-Media Services, BC Cancer Agency, 4Department of Pathology and Laboratory Medicine, University of British Columbia - UBC

This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

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