Gunma University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Low-Density Primary Hippocampal Neuron Culture

Reiko T. Roppongi^*1,2, Kevin P. Champagne-Jorgensen^*1,2, Tabrez J. Siddiqui^1,2

¹Department of Physiology and Pathophysiology, University of Manitoba, ²Kleysen Institute for Advanced Medicine, Health Sciences Centre

This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.

Education

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Kentaro Doi¹, Fumika Nito¹, Ayako Yano², Ryo Nagura¹, Satoyuki Kawano¹

¹Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, ²Division of Mechanical Science and Technology, Faculty of Science and Technology, GUNMA University

The rectification of ion transport pathways is an effective method to generate one-directional ion-dragged electrohydrodynamic flows. By setting an ion-exchange membrane in a flow channel, an electrically polarized condition is generated and causes a liquid flow to be driven when an electric field is externally applied.

Engineering

A Polymer-based Piezoelectric Vibration Energy Harvester with a 3D Meshed-Core Structure

Takuya Tsukamoto¹, Yohei Umino¹, Kotaro Hashikura¹, Sachie Shiomi¹, Kou Yamada¹, Takaaki Suzuki^1,2

¹Gunma University, ²JST PRESTO

In this study, we fabricated a flexible 3D mesh structure and applied it to the elastic layer of a bimorph cantilever-type vibration energy harvester for the purpose of lowering resonance frequency and increasing output power.

Behavior

Measurement of Spatial Stability in Precision Grip

Ryoto Teshima¹, Naoto Noguchi², Ryota Fujii³, Ken Kondo⁴, Koji Tanaka⁴, Bumsuk Lee⁴

¹Division of Rehabilitation Service, Geriatrics Research Institute and Hospital, ²Faculty of Rehabilitation, Gunma University of Health and Welfare, ³Department of Rehabilitation, Gunma Chuo Hospital, ⁴Graduate School of Health Sciences, Gunma University

The goal of this protocol is to measure the center of pressure (COP) replacement using a high spatial resolution sensor sheet to reflect the spatial stability in a precision grip. The use of this protocol could contribute to greater understanding of the physiology and pathophysiology of grasping.

Neuroscience

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Noriko Koganezawa¹, Reiko T. Roppongi², Yuko Sekino^3,4, Izuo Tsutsui³, Ayaka Higa⁵, Tomoaki Shirao^1,5

¹Department of Pharmacology, Graduate School of Medicine, Gunma University, ²Gunma University Initiative for Advanced Research, Gunma University, ³Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, ⁴Institute for Drug Discovery Innovation, ⁵AlzMed, Inc.

A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.