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Academy of Military Medical Sciences

4 ARTICLES PUBLISHED IN JoVE

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JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control
Charles E. Rupprecht 1, Wei-Cheng Hsu 2, Feng Ye 3, Megan Golding 4, Luca Zaeck 5, Laurent Dacheux 6, Noel Tordo 7, Jodie A. Jarvis 8, Erin Patrick 9, Are Berentsen 10
1LYSSA LLC, 2Animal Health Research Institute, Council of Agriculture, Executive Yuan, 3Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 4Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 5Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 6Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 7Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, Institut Pasteur de Guinea, Conakry, 8Rabies Laboratory, Wadsworth Center, New York State Department of Health, 9Animal and Plant Health Inspection Service, Wildlife Services, Knoxville, TN, US Department of Agriculture, 10Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, US Department of Agriculture

JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

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Developmental Biology

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
Lin-Chen Li *1,2, Xin-Xin Yu *1,2, Yu-Wei Zhang *1, Ye Feng *1,3, Wei-Lin Qiu *1,3, Cheng-Ran Xu 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University

We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

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Environment

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
Zhongyi Wang *1, Jiaming Li *2, Lu’an Qian *3, Linna Liu 1, Jun Qian 1, Bing Lu 1, Zhendong Guo 1
1Academy of Military Medical Sciences, 2Chinese Academy of Agricultural Sciences, 3The Experimental High School Attached to Beijing Normal University

Understanding the biological composition of environmental particulate matter is important for the study of its significant impacts on human health and disease spread. Here, we used three types of bioaerosol sampling methods and a biological analysis of airborne microbes to better explore airborne microbial communities under different environmental conditions.

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Genetics

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Yuyang Wang *1, Weidi Xu *1,2, Huancheng Guo 1, Wenjie Gong 1, Biao He 1, Zhongzhong Tu 1, Changchun Tu 1,3, Ye Feng 1
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

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