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Paris Descartes University

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
Elena Dossi 1, Thomas Blauwblomme 2,3, Rima Nabbout 2,4, Gilles Huberfeld 2,5, Nathalie Rouach 1
1Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, 2Infantile Epilepsies & Brain Plasticity, INSERM U1129, PRES, Paris Descartes University, Sorbonne Paris Cité, CEA, 3Neurosurgery Department, Necker Hospital, AP-HP, Paris Descartes University, 4Rare Epilepsies Reference Center, Necker Hospital, AP-HP, Paris Descartes University, 5Neurophysiology Department, La Pitié-Salpêtrière Hospital, AP-HP, Sorbonne and Pierre and Marie Curie University

We here describe how to perform multi-electrode array recordings of human epileptic cortical tissue. Epileptic tissue resection, slice preparation and multi-electrode array recordings of interictal and ictal events are demonstrated in detail.

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Cancer Research

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Clémence Granier 1, Emeline Vinatier 2,3,4, Elia Colin 2,3, Marion Mandavit 2,3, Charles Dariane 2,3,5, Virginie Verkarre 6, Lucie Biard 7, Rami El Zein 2, Corinne Lesaffre 2, Isabelle Galy-Fauroux 2, Hélène Roussel 2,3,6, Eléonore De Guillebon 8, Charlotte Blanc 2,3, Antonin Saldmann 4, Cécile Badoual 2,6, Alain Gey *4, Éric Tartour *2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou

Studying tumor microenvironment may identify prognostic or predictive biomarkers of clinical response to immunotherapy. Presented here, is an innovative method based on in situ fluorescence multispectral imaging to analyze and count automatically various subpopulations of CD8+ T cells. This reproducible and reliable technique is suitable for large cohort analyses.

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Genetics

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Sophie Outh-Gauer 1, Jérémy Augustin 1, Marion Mandavit 2, Ophélie Grard 2, Thomas Denize 1, Marine Nervo 1, Charles Lépine 1, Marc Rassy 2, Eric Tartour 2,3, Cécile Badoual 1,2
1Department of Pathology, Georges Pompidou European Hospital, Assistance publique - Hôpitaux de Paris (APHP), Paris Descartes University, 2Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, 3Laboratory of Immunology, Georges Pompidou European Hospital, APHP, Paris Descartes University

Human papillomavirus (HPV) RNA chromogenic in situ hybridization is considered to be one of the gold standards for active human papillomavirus infection detection within tumors. It allows the visualization of HPV E6-E7 mRNA expression with localization and semiquantitative evaluation of its signal.

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