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Sandia National Laboratories

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
Philip R. Miller 1,2, Shelby A. Skoog 1, Thayne L. Edwards 2, David R. Wheeler 2, Xiaoyin Xiao 2, Susan M. Brozik 2, Ronen Polsky 2, Roger J. Narayan 1
1Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, 2Department of Biosensors and Nanomaterials, Sandia National Laboratories

This article details the construction of a multiplexed microneedle-based sensor. The device is being developed for in situ sampling and electrochemical analysis of multiple analytes in a rapid and selective manner. We envision clinical medicine and biomedical research uses for these microneedle-based sensors.

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Bioengineering

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
Anupama Sinha 1, Mais J. Jebrail 2, Hanyoup Kim 2,3, Kamlesh D. Patel 4, Steven S. Branda 2
1Department of Systems Biology, Sandia National Laboratories, 2Department of Biotechnology and Bioengineering, Sandia National Laboratories, 3Canon U.S. Life Sciences, 4Department of Advanced Systems Engineering and Deployment, Sandia National Laboratories

We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or reagents.

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Engineering

Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes
Nils Hansen 1, Scott A. Skeen 1, Hope A. Michelsen 1, Kevin R. Wilson 2, Katharina Kohse-Höinghaus 3
1Combustion Research Facility, Sandia National Laboratories, 2Chemical Sciences Division, Advanced Light Source, Lawrence Berkeley National Laboratory, 3Physikalische Chemie I, Universität Bielefeld

Gas sampling from laboratory-scale flames with online analysis of all species by mass spectrometry is a powerful method to investigate the complex mixture of chemical compounds occurring during combustion processes. Coupled with tunable soft ionization via synchrotron-generated vacuum-ultraviolet radiation, this technique provides isomer-resolved information and potentially fragment-free mass spectra.

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Bioengineering

Forming Giant-sized Polymersomes Using Gel-assisted Rehydration
Adrienne C. Greene 1, Darryl Y. Sasaki 2, George D. Bachand 1
1Center for Integrated Nanotechnologies, Sandia National Laboratories, 2Biological and Engineering Sciences, Sandia National Laboratories

We present a protocol to rapidly form giant polymer vesicles (pGVs). Briefly, polymer solutions are dehydrated on dried agarose films adhered to coverslips. Rehydration of the polymer films results in rapid formation of pGVs. This method greatly advances the preparation of synthetic giant vesicles for direct applications in biomimetic studies.

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Bioengineering

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
Zachary I. Imam 1, George D. Bachand 1
1Center for Integrated Nanotechnologies, Sandia National Laboratories

This protocol describes a process for fabricating lipid nanotube networks using gliding kinesin motility in conjunction with giant unilamellar lipid vesicles.

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Engineering

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
Trevor Clark 1, Caitlin A. Taylor 1,2, Christopher M. Barr 1, Khalid Hattar 1
1Sandia National Laboratories, 2Los Alamos National Laboratory

Sample preparation techniques are outlined with specific considerations for in situ ion irradiation TEM experiments. Ion species, energy, and fluence are discussed with methods for how to select and compute them. Finally, procedures for conducting an experiment are described and accompanied by the representative results.

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Engineering

Co-localizing Kelvin Probe Force Microscopy with Other Microscopies and Spectroscopies: Selected Applications in Corrosion Characterization of Alloys
Olivia O. Maryon *1, Corey M. Efaw *1, Frank W. DelRio 2, Elton Graugnard 1,3, Michael F. Hurley 1,3, Paul H. Davis 1,3
1Micron School of Materials Science & Engineering, Boise State University, 2Material, Physical, and Chemical Sciences Center, Sandia National Laboratories, 3Center for Advanced Energy Studies

Kelvin probe force microscopy (KPFM) measures surface topography and differences in surface potential, while scanning electron microscopy (SEM) and associated spectroscopies can elucidate surface morphology, composition, crystallinity, and crystallographic orientation. Accordingly, the co-localization of SEM with KPFM can provide insight into the effects of nanoscale composition and surface structure on corrosion.

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