Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester MonomersSeamus Jenkin 1, Isabel Molina 1
1Department of Biology, Algoma University
Lipid polyesters constitute the structural components of two cell wall modifications, the plant cuticle and suberin-containing diffusion barriers. In this video, we describe a method to depolymerize cutin from whole delipidated leaves. The method can be applied to investigating mutants compromised in either cutin or suberin biosynthesis.