Universidade São Judas Tadeu

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats

Saeed Alshahrani¹, Filipe Fernandez-Conti², Amanda Araujo², Mauricio DiFulvio¹

¹Department of Pharmacology and Toxicology, Wright State University, ²Human Movement Laboratory, Universidade São Judas Tadeu

Here, we describe a rapid reliable and simple procedure to determine the lowest temperature at which rats or mice show nocifensive behavior, i.e. the thermal nociceptive threshold (TNT). This method applies a slowly increasing thermal stimulus allowing precise and reproducible estimation of TNTs with minimum, if any, stress to the animals.