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U.S. Army Medical Research Institute of Infectious Diseases

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines
Emily D. Cisney 1, Stefan Fernandez 1, Shannan I. Hall 1, Gale A. Krietz 1, Robert G. Ulrich 1
1U.S. Army Medical Research Institute of Infectious Diseases

Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.

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