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University Medical Center Freiburg

3 ARTICLES PUBLISHED IN JoVE

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Biology

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
Dominik Schnerch 1, Marie Follo 1, Julia Felthaus 1, Monika Engelhardt 1, Ralph Wäsch 1
1Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.

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Medicine

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Louise Benning 1, Samuel Robinson 1,2, Marie Follo 3, Lukas Andreas Heger 1, Daniela Stallmann 1, Daniel Duerschmied 1, Christoph Bode 1, Ingo Ahrens 1,4, Marcus Hortmann 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.

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Medicine

Erosion Identification in Metacarpophalangeal Joints in Rheumatoid Arthritis using High-Resolution Peripheral Quantitative Computed Tomography
Yousif Al-Khoury 1,2, Stephanie Finzel 3, Camille Figueiredo 4, Andrew J. Burghardt 5, Kathryn S. Stok 6, Lai-Shan Tam 7, Isaac Cheng 7, Justin J. Tse 1, Sarah L. Manske 1
1Department of Radiology, McCaig Institute for Bone and Joint Health, University of Calgary, 2Department of Biomedical Engineering, University of Calgary, 3University Medical Center Freiburg, 4Department of Rheumatology, University of São Paulo, 5Department of Radiology and Biomedical Imaging, University of California, San Francisco, 6Department of Biomedical Engineering, University of Melbourne, 7Department of Medicine and Therapeutics, The Chinese University of Hong Kong

Bone erosions are an important pathological feature of rheumatoid arthritis. The purpose of this work is to introduce a training tool to provide users with guidance on identifying pathological cortical breaks on high resolution peripheral quantitative computed tomography images for erosion analysis.

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