Office of Research and Development, US Environmental Protection Agency

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Sarah E. Staggs¹, Mary Jean See², J P. Dubey³, Eric N. Villegas^2,4

¹Dynamac, Inc., ²Department of Biological Sciences, University of Cincinnati, McMicken College of Arts and Science, ³Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, ⁴National Exposure Research Laboratory, US Environmental Protection Agency

This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation

Immunology and Infection

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Eric R. Rhodes¹, Leah Fohl Villegas², Nancy J. Shaw², Carrie Miller³, Eric N. Villegas¹

¹National Exposure Research Laboratory, Office of Research and Development, US Environmental Protection Agency, ²Shaw Environmental & Infrastructure, ³Office of Ground Water and Drinking Water, US Environmental Protection Agency

This protocol describes the use of a tangential flow hollow-fiber ultrafiltration sample concentration system and a heat dissociation as alternative steps for the detection of waterborne Cryptosporidium and Giardia species using EPA Method 1623.