University of Colorado Denver Anschutz Medical Campus

4 ARTICLES PUBLISHED IN JoVE

Biology

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

¹Department of Craniofacial Biology, University of Colorado Denver Anschutz Medical Campus, ²Department of Cell and Developmental Biology, University of Colorado Denver Anschutz Medical Campus

A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

Cancer Research

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Stacey Bagby¹, Wells A. Messersmith¹, Todd M. Pitts¹, Anna Capasso¹, Marileila Varella-Garcia¹, Peter J. Klauck¹, Jihye Kim¹, Aik-Choon Tan¹, S. Gail Eckhardt¹, John J. Tentler¹, John Arcaroli¹

¹Medicine, University of Colorado Denver Anschutz Medical Campus

Utilizing patient-derived tumors in a subcutaneous preclinical model is an excellent way to study the efficacy of novel therapies, predictive biomarker discovery, and drug resistant pathways. This model, in the drug development process, is essential in determining the fate of many novel anti-cancer therapies prior to clinical investigation.

Education

Collecting Hair Samples for Hair Cortisol Analysis in African Americans

Kathy D. Wright¹, Jodi L. Ford¹, Joseph Perazzo², Lenette M. Jones³, Sherrilynn Mahari⁴, Brent A. Sullenbarger^5,6,7, Mark L. Laudenslager⁸

¹College of Nursing, The Ohio State University, ²College of Nursing, University of Cincinnati, ³School of Nursing, University of Michigan, ⁴Licensed Cosmetologist Consultant, ⁵Clinical Research Manager, The Ohio State University, ⁶Biomedical Core Research Coordinator, The Ohio State University, ⁷College of Nursing Biomedical Core Research Laboratory, The Ohio State University, ⁸Department of Psychiatry, Behavioral Immunology & Endocrinology Laboratory, University of Colorado Denver Anschutz Medical Campus

Hair cortisol concentration analysis provides an alternative to traditional measures of cortisol; however, to collect hair samples from African Americans, scientists need to be culturally informed and competent. The purpose of this protocol is to demonstrate a culturally informed technique to collect hair samples for cortisol analysis from African Americans.

Cancer Research

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Jordi M. Lanis^*1, Matthew S. Lewis^*1, Hannah Strassburger¹, Kristina Larsen¹, Stacey M. Bagby², Adrian T. A. Dominguez², Juan A. Marín-Jiménez³, Roberta Pelanda¹, Todd M. Pitts², Julie Lang¹

¹Department of Immunology and Microbiology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, ²Division of Oncology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, ³Department of Medical Oncology, Catalan Institute of Oncology (ICO-L'Hospitalet)

This protocol outlines the generation of human immune system (HIS) mice for immuno-oncology studies. Instructions and considerations in the use of this model for testing human immunotherapeutics on human tumors implanted in this model are presented with an emphasis on characterizing the response of the human immune system to the tumor.