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Vanderbilt University Medical School

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Gleb Pishchany 1, Kathryn P. Haley 1, Eric P. Skaar 1
1Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical School

Here we describe a growth assay for Staphylococcus aureus using hemoglobin as the sole source of available nutrient iron. This assay establishes the role of bacterial factors involved in hemoglobin-derived iron acquisition.

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Developmental Biology

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
Chen Huang 1, Guoqiang Gu 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.

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Biochemistry

Expression, Purification, and Antimicrobial Activity of S100A12
Emmanuel Jackson 1, Saffron Little 1, Dana S. Franklin 1, Jennifer A. Gaddy 2,3, Steven M. Damo 1,4
1Department of Life and Physical Sciences, Fisk University, 2Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs, 3Department of Medicine - Division of Infectious Diseases, Vanderbilt University Medical School, 4Department of Biochemistry, Vanderbilt University

Here, we present a method to express and purify S100A12 (calgranulin C). We describe a protocol to measure its antimicrobial activity against the human pathogen H. pylori.

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