Brown University - National Institutes of Health Graduate Partnership Program

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

Vania Y. Cao^1,2, Yizhou Ye¹, Surjeet S. Mastwal¹, David M. Lovinger³, Rui M. Costa⁴, Kuan H. Wang¹

¹Unit on Neural Circuits and Adaptive Behaviors, Genes Cognition and Psychosis Program, National Institute of Mental Health, ²Department of Neuroscience, Brown University - National Institutes of Health Graduate Partnership Program, ³Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, ⁴Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown

Experience-dependent molecular changes in neurons are essential for the brain's ability to adapt in response to behavioral challenges. An in vivo two-photon imaging method is described here that allows the tracking of such molecular changes in individual cortical neurons through genetically encoded reporters.

Behavior

Multi-layer Cortical Ca²⁺ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Srishti Gulati¹, Vania Y. Cao¹, Stephani Otte¹

¹Inscopix Inc.

Here, we present a procedure for performing large-scale Ca²⁺ imaging with cellular-resolution across multiple cortical layers in freely moving mice. Hundreds of active cells can be observed simultaneously using a miniature, head-mounted microscope coupled with an implanted prism probe.