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Centre de Recherche Public-Gabriel Lippmann

2 ARTICLES PUBLISHED IN JoVE

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Biology

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.
Matias Pasquali 1,2, Corby Kistler 3
1Cereal Disease Laboratory, USDA, 2University of Minnesota/ Agroinnova, University of Torino, 3Cereal Disease Laboratory, University of Minnesota

To study the developmental processes of ascospores in Gibberella zeae, a procedure for collection under sterile conditions is filmed in order to generate the highest level of information for protocol description. This should facilitate the reproducibility of the experiment, a crucial aspect when full genome expression profile tests are implemented.

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Biology

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Matias Pasquali 1, Frédéric Giraud 1, Jean Paul Lasserre 1, Sebastien Planchon 1, Lucien Hoffmann 1, Torsten Bohn 1, Jenny Renaut 1
1Department of Environment and Agro-Biotechnologies (EVA), Nutrition and Toxicology Unit (NuTox), Centre de Recherche Public-Gabriel Lippmann

Protein extraction for proteomic analyses in fungal species requires high levels of standardization to be accomplished according with the minimum information about a proteomic experiment (MIAPE) guidelines. We present a video-protocol that includes a procedure for minimizing experimental bias during toxin induction and protein extraction from Fusarium spp.

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