JoVE Logo

Sign In

The Samuel Roberts Noble Foundation

2 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Muthappa Senthil-Kumar 1, Hee-Kyung Lee 1, Kirankumar S. Mysore 1
1Plant Biology Division, The Samuel Roberts Noble Foundation

Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.

image

Biology

Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology
Vadivelmurugan Irulappan 1, Muthappa Senthil-Kumar 1
1National Institute of Plant Genome Research

This study presents methodologies to study the pathomorphological and molecular mechanisms underlying chickpea–Rhizoctonia bataticola interaction. The blotting paper method is useful to rapidly study chickpea genotype responses, while the sick pot-based method can be used to simultaneously impose drought and R. bataticola infection and screen for tolerant genotypes.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved