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University Medical Center Mainz

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
J. Moritz Kaths 1,2, Vinzent N. Spetzler 1, Nicolas Goldaracena 1, Juan Echeverri 1, Kristine S. Louis 1, Daniel B. Foltys 3, Mari Strempel 4, Paul Yip 5, Rohan John 5, Istvan Mucsi 1, Anand Ghanekar 1, Darius Bagli 6,7, Lisa Robinson 2, Markus Selzner 1
1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to  transplantation.

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Immunology and Infection

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls
Jeremy Lagrange 1, Sabine Kossmann 1,2, Klytaimnistra Kiouptsi 1, Philip Wenzel 1,2,3
1Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, 2Center for Cardiology, University Medical Center Mainz, 3German Center for Cardiovascular Research (DZHK)

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

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Neuroscience

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound
Axel Neulen *1, Michael Molitor *2,3,4, Michael Kosterhon *1, Tobias Pantel 1, Susanne H. Karbach 2,3,4, Philip Wenzel 2,3,4, Thomas Gaul 5, Florian Ringel 1, Serge C. Thal 5
1Department of Neurosurgery, University Medical Center of the Johannes Gutenberg-University of Mainz, 2Center for Cardiology - Cardiology I, University Medical Center of the Johannes Gutenberg-University of Mainz, 3Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University of Mainz, 4German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, 5Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University of Mainz

The aim of this manuscript is to present a sonography-based method that allows in vivo imaging of blood flow in cerebral arteries in mice. We demonstrate its application to determine changes in blood flow velocities associated with vasospasm in murine models of subarachnoid hemorrhage (SAH).

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