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Boston University Photonics Center

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

In vivo Neuronal Calcium Imaging in C. elegans
Samuel H. Chung *1,2, Lin Sun *1,2, Christopher V. Gabel 1,2
1Department of Physiology and Biophysics, Boston University School of Medicine, 2Boston University Photonics Center

With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.

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Immunology and Infection

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Lin Sun 1, Naoko Kono 2, Hiroyuki Toh 3, Hanbing Xue 1, Kaori Sano 4,5, Tadaki Suzuki 4, Akira Ainai 4, Yasuko Orba 6, Junya Yamagishi 7,8, Hideki Hasegawa 4,5, Yoshimasa Takahashi 9, Shigeyuki Itamura 2, Kazuo Ohnishi 9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

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Medicine

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration
Jin Wang *1, Wenjia Li *2, Yao He 1, Yuanbin Xia 1, Lin Sun 1, Youpeng Zhang 1, Zhaohui Zhu 1
1Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

This protocol presents the clinical application of a 24 G cannula and 3-0 polypropylene suture as a simple and effective method for the exploration of the vas deferens.

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