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Kansai Medical University

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Preparing Activated Platelet-Rich Plasma for Culturing Human Adipose-Derived Stem Cells
Fangyuan Lai 1,2, Natsuko Kakudo 1, Naoki Morimoto 1, Yuanyuan Ma 1, Kenji Kusumoto 1
1Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2Department of Plastic Surgery, Zhejiang Provincial People's Hospital

In this report, we describe a double-spin method for preparing activated platelet-rich plasma (PRP). Using autologous thrombin, human adipose-derived stem cells (hASCs) were cultured. Activated PRP was shown to promote proliferation of hASCs.

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Bioengineering

A Novel Cell Injection Method with Minimum Invasion
Fumiyuki Hattori 1
1Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University

This method eliminates any major invasion during cell injections caused by the cell suspension solution.

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Biology

Application of Live Mitochondria Staining in Cell-Sorting to Purify Hepatocytes Derived from Human Induced Pluripotent Stem Cells
Hiromi Yamashita 1, Fumiyuki Hattori 1
1Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University

Hepatocytes derived from pluripotent stem cells can be purified through cell sorting, using a combination of mitochondrial and activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) staining.

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