National Research Council (CNR)

2 ARTICLES PUBLISHED IN JoVE

Biology

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Elisa Di Pasquale^1,2, Belle Song¹, Gianluigi Condorelli¹

¹Humanitas Clinical and Research Center, Italy, ²Institute of Genetic and Biomedical Research (IRGB), National Research Council (CNR)

Pluripotent stem cells, either embryonic or induced pluripotent stem (iPS) cells, constitute a valuable source of human differentiated cells, including cardiomyocytes. Here, we will focus on cardiac induction of iPS cells, showing how to use them to obtain functional human cardiomyocytes through an embryoid bodies-based protocol.

Biology

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Matteo Fossati^1,2,3, Nica Borgese^2,3,4, Sara Francesca Colombo^2,3, Maura Francolini^1,2

¹Fondazione Filarete, ²Department of Biotechnology and Translational Medicine, University of Milan, ³Neuroscience Institute, National Research Council (CNR), ⁴Department of Health Science, "Magna Graecia" University of Catanzaro

We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.